Behavior of solid and ascites sublines of two tumors in tissue culture.

METHODS AJÕ the tumors1 were received as subcutaneous transplants on OSH mice. On the llth day after inoculation the animals were sacrificed, and the tumors were explanted under sterile conditions. The cultures .were explanted in Carrel flasks, roller tubes, or coverslips. The best .results were obtained with expiants cultivated in Carrel flasks. Depending on the method used there were differ ences in the intensity of growth; the growth type, however, remained the same. . The experiments described in this paper were conducted in Carrel flasks. Thà ẗumors studied in this investigation were the following: MCIM/AS, MCIM/SS, MCIA/AS,, MCIA/SS. Ten samples of each of the tumors were used for each transfer. The tumor-bearing animals were killed, and the skin covering the tumors was dehaired with sulfide and then treated with medicinal iodine. The tumors were carefully excised, and ten very thin slices of each, measuring approximately 1X2 mm, were immersed into Tyrode solution. Single expiants were placed on the bottom of a Carrel flask together with a drop of chicken plasma and fixed with the aid of a cataract knife. To accelerate the coagulation of the plasma, the drop was then touched with the tip of a knife previously treated